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KMID : 0357419930230010057
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Korean journal of Virology
1993 Volume.23 No. 1 p.57 ~ p.68
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Abstract
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Our previous results have been demonstrated that Maaji viral cDNA was able to be amplified as well by the primers specific to Hantaan virus which was built for the purpose of differentiation of Hantaan virus from other serotypes of hantavirus by
amplification of viral cDNA by polymerase chain reaction(PCR). We conjectured the results as a cross amplification because the PCR pruducts was recognizable by difference of the size and of the restriction profiles.
In order to make the difference between two evident, nucleotide sequencing of amplified Maaji viral cDNA fragment was carried out. Amplification was done by PCR system using Taq polymerase. Sequencing of amplified Hantaan and Maaji viral cDNA
fragments
were carried out by Circum Vent sequencing method which is simpler and rather effective to ds DNA fragment like as PCR products. The analysis of nucleotide sequence indicated that the percent of homology between amplified region of Hantaan and
Maaji
virus was 82.4%. The difference of restriction profiles be tween Hantaan and Maaji viral cDNA fragments which have been demonstrated perviously could be explained by this sequence analysis.
As an evidence for crose amplification of Maaji viral cDNA by Hantaan specific primers, it was found recently that Vent DNA polymerase used as a substitute of Taq polymerase amplifies specifically Hantaan viral cDNA but not Maaji viral cDNA.
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